Journal: Tissue Engineering. Part C, Methods
Article Title: SIMPoly: A Matlab-Based Image Analysis Tool to Measure Electrospun Polymer Scaffold Fiber Diameter
doi: 10.1089/ten.tec.2020.0304
Figure Lengend Snippet: Diagram of the overall workflow in the SIMPoly fiber analysis algorithm. Starting with an original image (A) , image processing steps include contrast enhancement through histogram equalization (B) , reconstruction to brighten desired fibers (C) , edge overlay for later background noise removal (D) , thresholding for initial removal of background noise (E) , image dilation and erosion for “closing” to correct any gaps created from previous filtering step (F) , median filter application to smooth fiber edges and remove any leftover noise (G) , dilation of fibers to brighten edges to normal size without presence of background noise (H) , and skeletonization of image and overlay of skeleton onto the binary image (I) . Color images are available online.
Article Snippet: In addition, three researchers individually measured every image from the PLGA image set by using the new Matlab-based SIMPoly tool.
Techniques: